Steps for submission:

1. Check that GEO accepts your data type.
2. Gather raw files.
3. Gather processed data files.
4. Download metadata spreadsheet and fill in Metadata tab for your study. Use one spreadsheet per data type (e.g., ChIP-seq, RNA-seq).
5. In the metadata spreadsheet file, list the MD5 checksum for all raw and processed data files in the 'MD5 Checksums' tab.
6. Create single folder on your computer that contains all raw and processed data files for your experiment. If you have multiple data types, please use one folder per experiment.
7. Transfer your data to GEO by FTP using these instructions.
8. After FTP transfer has completed, submit metadata file(s) on the Submit to GEO page.

Download required spreadsheets:

• Metadata spreadsheet
• Metadata spreadsheet with SRA accessions

GEO provides the following video tutorial to assist with submission: 

• How To Submit High-Throughput Sequence Data to GEO